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Discussion papers
https://doi.org/10.5194/soil-2019-50
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/soil-2019-50
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

Submitted as: original research article 21 Aug 2019

Submitted as: original research article | 21 Aug 2019

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This discussion paper is a preprint. It is a manuscript under review for the journal SOIL (SOIL).

Adsorption to soils and biochemical characterization of purified phytases

Maria Marta Caffaro1,2, Karina Beatriz Balestrasse1,3, and Gerardo Rubio1,2 Maria Marta Caffaro et al.
  • 1INBA, CONICET UBA, Buenos Aires, C1417DSE, Argentina
  • 2Soil Fertility and Fertilizers, School of Agriculture University of Buenos Aires, Buenos Aires, C1417DSE, Argentina
  • 3Biochemistry, School of Agriculture University of Buenos Aires, Buenos Aires, C1417DSE, Argentina

Abstract. Four purified phytases isolated from Aspergillus niger and Escherichia coli were characterized biochemically and in terms of their adsorption to soils belonging to the Mollisol order. Three different organic P substrates were used to measure enzyme activity in a wide range of pH (2.3 to 9) and temperatures (−10° to 70 °C): p-nitrophenyl-phosphate, glyceraldehyde-3-phosphate and phytic acid. Phytases from A. niger showed a higher capacity to release P (36 to 50 % of P contained in the substrates, 44 to 62 μg P), than phytases from E. coli (24 to 36 %, 20 to 44 μg P). The amount of P released from organic P substrates by A. niger phytases followed the following range: p-nitrophenyl-phosphate > glyceraldehyde-3-phosphate > phytic acid whereas in E. coli phytases the order was p-nitrophenyl-phosphate/glyceraldehyde-3-phosphate > phytic acid. All phytases were active throughout the pH and temperature ranges for optimum crop production. The proportion of phytases found in the solid phase of the soil 60 minutes after addition was lower than that found in the liquid phase (23–34 % vs. 66–77 %). Obtained results are promising in terms of the use of phytases as a complement to P fertilization in agricultural settings and encourages further studies under field conditions.

Maria Marta Caffaro et al.
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Maria Marta Caffaro et al.
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Short summary
The prospects of using purified phytases isolated from Aspergillus niger and Escherichia coli as biological fertilizers were evaluated under controlled conditions. All of them were able to release inorganic P from organic sources and were active throughout the pH and temperature ranges for optimum crop production. Obtained results are promising in terms of the use of phytases as a complement to P fertilization in agricultural settings.
The prospects of using purified phytases isolated from Aspergillus niger and Escherichia coli as...
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