Journal cover Journal topic
SOIL An interactive open-access journal of the European Geosciences Union
Journal topic

Journal metrics

Journal metrics

  •  value: indexed indexed
  • CiteScore value: 5.79 CiteScore
    5.79
  • SNIP value: 2.052 SNIP 2.052
  • IPP value: 5.30 IPP 5.30
  • SJR value: 1.943 SJR 1.943
  • Scimago H <br class='hide-on-tablet hide-on-mobile'>index value: 21 Scimago H
    index 21
  • h5-index value: 22 h5-index 22
Discussion papers
https://doi.org/10.5194/soild-2-1135-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/soild-2-1135-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Submitted as: original research article 30 Oct 2015

Submitted as: original research article | 30 Oct 2015

Review status
This discussion paper is a preprint. It has been under review for the journal SOIL (SOIL). The manuscript was not accepted for further review after discussion.

Compound-specific 15N stable isotope probing of N assimilation by the soil microbial biomass: a new methodological paradigm in soil N cycling

A. F. Charteris1, T. D. J. Knowles1, K. Michaelides2, and R. P. Evershed1 A. F. Charteris et al.
  • 1Organic Geochemistry Unit, School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK
  • 2School of Geographical Sciences, University of Bristol, University Road, Bristol, BS8 1SS, UK

Abstract. A compound-specific nitrogen-15 stable isotope probing (15N-SIP) technique is described which allows investigation of the fate of inorganic- or organic-N amendments to soils. The technique uses gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) to determine the δ15N values of individual amino acids (AAs; determined as N-acetyl, O-isopropyl derivatives) as proxies of biomass protein production. The δ15N values are used together with AA concentrations to quantify N assimilation of 15N-labelled substrates by the soil microbial biomass. The utility of the approach is demonstrated through incubation experiments using inorganic 15N-labelled substrates ammonium (15NH4+) and nitrate (15NO3-) and an organic 15N-labelled substrate, glutamic acid (15N-Glu). Assimilation of all the applied substrates was undetectable based on bulk soil properties, i.e. % total N (% TN), bulk soil N isotope composition and AA concentrations, all of which remained relatively constant throughout the incubation experiments. In contrast, compound-specific AA δ15N values were highly sensitive to N assimilation, providing qualitative and quantitative insights into the cycling and fate of the applied 15N-labelled substrates. The utility of this 15N-AA-SIP technique is considered in relation to other currently available methods for investigating the microbially-mediated assimilation of nitrogenous substrates into the soil organic N pool. This approach will be generally applicable to the study of N cycling in any soil, or indeed, in any complex ecosystem.

A. F. Charteris et al.

Interactive discussion

Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement

Interactive discussion

Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement

A. F. Charteris et al.

A. F. Charteris et al.

Viewed

Total article views: 916 (including HTML, PDF, and XML)
HTML PDF XML Total BibTeX EndNote
595 290 31 916 33 28
  • HTML: 595
  • PDF: 290
  • XML: 31
  • Total: 916
  • BibTeX: 33
  • EndNote: 28
Views and downloads (calculated since 30 Oct 2015)
Cumulative views and downloads (calculated since 30 Oct 2015)

Cited

Saved

Discussed

No discussed metrics found.
Latest update: 23 Feb 2020
Publications Copernicus
Download
Citation